Yahia, M.Y.A.; Hassan, M.M.; Elamin, M.A.; Abdalla, N.K.; Rugheim, A.M.E.
; Osman, A.G.
and Babiker, A.G.T. ,Sudan
A series of field, laboratory and greenhouse experiments were conducted at the Bio-pesticides
and Bio-fertilizers Department, Environment, Natural Resources and Desertification Research
Institute (ENDRI), National Centre for Research (NCR), Khartoum, Sudan and College of
Agricultural Studies, Sudan University of Science and Technology at Shambat, Khartoum North
Sudan to study the effects of Trichoderma harzianum, herbicide imazethapyr, Bacillus
megatherium var. phosphaticum (BMP) and Rhizobium leguminosarum biovar viceae strain
(TAL1399) on O. crenata germination, incidence and faba bean growth, nodules number and
plant height. Results of germination experiment showed that application of GR24 to O. crenata
seeds conditioned in water induced germination to 80%. Application of T. harzianum
significantly reduced O. crenata germination by 82% compared to control. The combination
BMP + TAL1399 significantly reduced O. crenata germination by 32-42% as compared to
control. Destructive sample experiment results showed that T. harzianum alone gave the highest
nodules number and nodules dry weight compared to the control and the herbicide. The bacterial
strains BMP and TAL1399 each alone or in combination significantly increased nodules number
and dry weight as compared to the control. Extract of nodules collected from roots treated with
T. harzianum and imazethapyr each alone or in combination, BMP and TAL1399 each alone or
in combination significantly decreased O. crenata seed germination as compared to the control.
Greenhouse results showed that T. harzianum and imazethapyr alone or in combination
significantly decreased nodules numbers and O. crenata numbers as compared to the infected
control and significantly increased faba bean biomass and plant height as compared to control.
BMP, TAL1399 each alone or in combination significantly increased plant height, faba bean
biomass and nodules numbers and decreased O. crenata number as compared to the infested