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ISSN : 2456-8643

Title:
MICRO SATELLITE MARKERS SCREENING FOR COFFEE BERRY DISEASE (COLLETOTRICHUM KAHAWAE) RESISTANCE IN PROGENIES OF VARIETY KP423 AND ETHIOPIAN COFFEE ARABICA ACCESSIONS

Authors:
MTENGA, Damian J ., KUSOLWA, Paul M., REUBEN, Shazia. O.W.M and KILAMBO, Deusdedit L. ,Tanzania

Abstract:
Coffee berry disease (CBD), caused by Colletotrichum kahawae, is a major constraint for Arabica coffee cultivation in Africa. Several previous studies have revealed molecular markers associated with its resistance. CBD is a disease that attacks berries at different developmental stages. The current study was aimed at using of these markers to screen for resistance to the disease on genotypes and progenies developed from the Tanzanian commercial variety and Ethiopian accessions at seedling stage. Eleven Ethiopian genotypes were crossed to a variety KP423. F1 progenies and their parental genotypes were used in the study. Physiological screening was applied on the hypocotyls of parental genotypes and F1 progenies using the procedure developed by Van der Vossen in 1976. Marker screening was applied on the DNA extracted from their young tender leaves using gene specific markers Sat 235 and Sat 207. The presence of the coffee berry disease resistance genes was revealed in the studied coffee genotypes amplified by SSR marker Sat 235 and Sat 207. This was confirmed by production of bands similar to the progenitors of CBD resistance. These findings implied that marker screening can be used in coffee berry disease resistant genotypes selection at early stages of growth hence reducing the time of selection cycle.

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